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human fibroblast growth factor fgf-10  (PeproTech)


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    PeproTech human fibroblast growth factor fgf-10
    Human Fibroblast Growth Factor Fgf 10, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fibroblast+growth+factor-10+(fgf-10)/fgf10+growth+factor/us12351829-528-44-50
    Average 90 stars, based on 1 article reviews
    human fibroblast growth factor fgf-10 - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: IL-6-mediated tumorigenicity and antioxidant state in squamous cell carcinoma cells are driven by CD109 via stabilization of IL-6 receptor-alpha and activation of STAT3/NRF2 pathway.
    Article Snippet: Tumor spheroid formation assay Single cell suspensions (10,000 cells) of A431 or SCC9 WT and their corresponding CD109 KO or A431 empty vector (EVA431) or A431 overexpressing CD109 (OECD109 A431) cells were suspended in six-well ultra-low attachment plate (VWR; #29443-030) in DMEM/F12 Medium (Gibco; #11320033) with 20 ng/ml recombinant human fibroblast growth factor10 (FGF-10) (PeproTech; #100- 26), 20 ng/ml hFGF2 (PeproTech; #100-18B), 9.5 μg/ml insulin (Sigma-Aldrich; # I9278) and 2% Gibco B-27 Supplement (ThermoFisher Scientific; #17504-044) at 37 °C in 5% CO2.

    Article Title: IL-6-mediated tumorigenicity and antioxidant state in squamous cell carcinoma cells are driven by CD109 via stabilization of IL-6 receptor-alpha and activation of STAT3/NRF2 pathway
    Article Snippet: Single cell suspensions (10,000 cells) of A431 or SCC9 WT and their corresponding CD109 KO or A431 empty vector (EVA431) or A431 overexpressing CD109 (OECD109 A431) cells were suspended in six-well ultra-low attachment plate (VWR; #29443-030) in DMEM/F12 Medium (Gibco; #11320033) with 20 ng/ml recombinant human fibroblast growth factor10 (FGF-10) (PeproTech; #100-26), 20 ng/ml hFGF2 (PeproTech; #100-18B), 9.5 μg/ml insulin (Sigma-Aldrich; # I9278) and 2% Gibco B-27 Supplement (ThermoFisher Scientific; #17504-044) at 37 °C in 5% CO 2 .

    Article Title: MYC is a regulator of androgen receptor inhibition-induced metabolic requirements in prostate cancer
    Article Snippet: Human fibroblast growth factor (FGF)-10 , PeproTech , Cat#100–26.

    Article Title: Generation, proliferation and expansion of epithelial cells from primary tissue into mucosoid cultures
    Article Snippet: Composition of the Media Advanced DMEM/F12 (ADF, GIBCO), 50% conditioned WNT3A-medium and 25% conditioned R-spondinl medium 24 supplemented with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 1% Glutamax, 2% B27, 1% N2, 20 ng/ml human epidermal growth factor (EGF) (all Invitrogen), 150 ng/ml human noggin, 150 ng/ml human fibroblast growth factor (FGF)-10 (both Peprotech), 10 mM nicotinamide, 10 nM human gastrin, 2 μM SB202190, 9 μM Y-27632, 1 nM CHIR99021 and 1 μM PGE2 (all Sigma) 1 μM A83-01 (Calbiochem).

    Article Title: CYLD-TRAF6 interaction promotes ADP-heptose-induced NF-κB signaling in H. pylori infection.
    Article Snippet: D-MEM/F12, Thermo Fisher Scientific, 12634-010) supplemented with 10mM HEPES (Thermo Fisher Scientific, 15630056), 1% Glutamax (Thermo Fisher Scientific, 35050038), 1% (v/v) penicillin/streptomycin (Thermo Fisher Scientific, 15140-122), 2% B27 supplement (Thermo Fisher Scientific, 17504-044), 1% N2 supplement (Thermo Fisher Scientific, 17502-048), 20 ng/ml human epidermal growth factor (EGF) (Thermo Fisher Scientific, PHG0311), 25% conditioned R-spondin medium, 25 ng/ml Wnt surrogate-Fc fusion protein (ImmunoPrecise Antibodies Ltd, N001-0.1 mg), 150 ng/ml human noggin (Peprotech, 120-10C), 150 ng/ml human fibroblast growth factor (FGF)-10 (Peprotech, 100-26), 1.25 mM N-acetyl-L-cysteine (Sigma-Aldrich, A9165), 10mM nicotinamide (Sigma-Aldrich, N0636-100G), 10 nM human [Leu15]-Gastrin I (Sigma-Aldrich, G9145), 7.5 μM ROCK inhibitor (Y-27632) (Sigma-Aldrich, Y0503) and 1 μM TGF-β RI Kinase Inhibitor IV (Alk-I) (Calbiochem, 616454), and incubated at 37 °C, 5% CO2 in a humidified incubator.

    Article Title: The Fragile X Messenger Ribonucleoprotein 1 Regulates the Morphology and Maturation of Human and Rat Oligodendrocytes
    Article Snippet: Spheres were further expanded in the presence of basic fibroblast growth factor (FGF)‐2 (10 ng/mL, 45033, PeproTech) for 7 days, following which neural differentiation was induced by FGF2 withdrawal for a further 2 weeks.

    Article Title: Digital PCR characterizes epithelial cell populations in murine duodenal organoids.
    Article Snippet: Complete organoid culture medium requires basal medium, added with 50% conditioned Wnt3A-medium and 10% conditioned R-spondin1 medium, and supplemented with 20 ng/ ml murine epidermal growth factor (Invitrogen, PMG8043), 150 ng/ml murine noggin (Peprotech, 250-38), 150 ng/mL human fibroblast growth factor (FGF)-10 (Peprotech, 100- 26), 1.25 mM N-acetyl-L-cystein, 10mM nicotinamide, 1 mM TGF-β RI Kinase Inhibitor IV (Calbiochem, A83–01).

    Concentration Assay:

    Article Title: Regulatory T cell depletion promotes myeloid cell activation and glioblastoma response to anti-PD1 and tumor-targeting antibodies.
    Article Snippet: Cell lines and tissue culture NSC-derived tumor cell lines NPE-IE (kindly given by S. Pollard, University of Edinburgh) were grown using NeuroCult Basal Medium (Mouse & Rat, Cat #05700, StemCell Technologies) supplemented with NeuroCult Proliferation Supplement (Cat #05701; StemCell Technologies), 100 UI/mL penicillin, 100 mg/mL streptomycin (Cat #15140-122, Gibco). .. This medium was supplemented withmouse epidermal growth factor (EGF, final concentration 10 ng/mL; Cat #315-09, Peprotech) and human fibroblast growth factor (FGF, final concentration 10 ng/mL; Cat #100-18B, Peprotech) immediately prior to use. ..



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    PeproTech human fibroblast growth factor fgf-10
    Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with <t>recombinant</t> C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric <t>fibroblast</t> co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).
    Human Fibroblast Growth Factor Fgf 10, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with recombinant C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric fibroblast co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).

    Journal: Cellular and Molecular Gastroenterology and Hepatology

    Article Title: Helicobacter pylori -Induced Inflammatory Cancer Transformation Microenvironment Drives Gastric Carcinogenesis

    doi: 10.1016/j.jcmgh.2026.101757

    Figure Lengend Snippet: Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with recombinant C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric fibroblast co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).

    Article Snippet: Exactly 5000 viable cells per well were seeded in sphere formation medium consisting of DMEM/F12 supplemented with 2% B27 Supplement, 20 ng/mL recombinant human epidermal growth factor (EGF), and 10 ng/mL recombinant human fibroblast growth factor (FGF) (all Gibco).

    Techniques: Biomarker Discovery, Tube Formation Assay, Migration, Recombinant, Control, Co-Culture Assay, Cell Culture, Staining, Immunofluorescence